Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

MetaSystems是一家雇员持股的德国公司，专门从事自动扫描和成像系统的开发，用于细胞遗传学,癌症遗传学、毒理学、病理学、法医科学和其他应用领域。
 
发展史简介
1986年 --- 由德国海德堡大学应用物理研究所技术转移而成立，专注于细胞遗传学及分子遗传学自动化图像工作站之研发
1987年 --- 第一套Metafer系列之MSearch分裂中期细胞扫描仪安装完成
1991年 --- 第一套Ikaros染色体核型分析工作站安装完成
1994年 --- 发表ISIS荧光原位杂交图像分析工作站
1997年 --- 第一套Metafer系列之RCDetect罕有细胞自动扫描仪安装完成发表mFISH软件

1998年 --- 发表24XCyte mFISH荧光探针及mBAND荧光探针
2000年 --- 发表Metafer系列新成员- MetaCyte间期细胞荧光点扫描仪
2004年 --- 成立MetaSystems Aisa公司负责亚洲地区业务推广及技术支持
2006年 --- 在全球近70个国家800多个实验室中已安装超过2000套工作站，成为欧州及澳州地区之领导品牌。
2009/2010年 --- 投入研发自动化虚拟幻灯片成像系统
在大力开发成像系统的同时，Mestasystem也逐步扩增其探针产品系列，如全染色体涂染试剂盒（XCP）、小鼠和大鼠多色mFISH探针（21XMouse和22XRat）、染色体臂特异性mFISH探针(XaCyte)等。早在2009年MetaSystems就曾引进新系列用于血液学研究的染色体特殊位点探针。



Metasystems公司FISH 探针产品系列


1. XL XCyting Locus-Specific Probe Kits位点特异性探针

血液学/肿瘤学荧光原位杂交探针
Chronic Myeloproliferative Disorders/  慢性骨髓增生性疾病	Myelodysplastic Syndromes/  骨髓增生异常综合症	Burkitt Lymphoma/  伯基特淋巴瘤
CLL (Chronic Lymphocytic Leukemia)/  慢性淋巴细胞白血病	AML (Acute Myeloid Leukemia)/  急性骨髓性白血病	Diff. Large B-Cell Lymphoma/  弥漫性大B淋巴瘤
ALL (Acute Lymphocytic Leukemia)/  急性淋巴细胞性白血病	Multiple Myeloma/   多发性骨髓瘤	Follicular Lymphoma/  滤泡型淋巴瘤
Mantle Cell Lymphoma/  套细胞淋巴瘤


2. XA XCyting AneuScore Probe Kits

3. XCP XCyting Chromosome Paints


XCyting Whole Chromosome Paints/全染色体涂染探针
Human Chromosomes/人全染色体涂染探针	Mouse and Rat Chromosomes/小鼠及大鼠染色体涂染探针
XCyting 染色体臂特异性探针


4.XCE XCyting Centromere Enumeration Probes

5.XCyting Multicolor DNA Probe Kits


人类染色体多色FISH探针试剂盒
24XCyte mFISH Probe Kit	Arm-Specific mFISH Probe Kit/染色体臂特异性mFISH探针	Centromeric mFISH Kit/着丝粒mFISH探针
小鼠和大鼠染色体多色FISH探针试剂盒